Journal: Adipocyte

Article Title: High-fat diet-induced obesity Rat model: a comparison between Wistar and Sprague-Dawley Rat

doi: 10.1080/21623945.2015.1061723

Figure Lengend Snippet: Primer sequences and real-time PCR conditions used for gut microbiota analysis

Article Snippet: Data are presented as the mean values of duplicate PCR analysis. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Target group Primer sequence (5′-3′) Genomic DNA Standard PCR product Size (bp) AT Reference Firmicutes ATG TGG TTT AAT TCG AAG CA AGC TGA CGA CAA CCA TGC AC Lactobacillus gasseri ATCC 33323 126 60°C 38 Bacteroidetes CAT GTG GTT TAA TTC GAT GAT AGC TGA CGA CAA CCA TGC AG Bacteroides vulgatus ATCC 8482 126 60°C 38 Lactobacillus GAG GCA GCA GTA GGG AAT CTT C GGC CAG TTA CTA CCT CTA TCC TTC TTC Lactobacillus gasseri ATCC 33323 126 60°C 39 Enterococcus CCC TTA TTG TTA GTT GCC ATC ATT ACT CGT TGT ACT TCC CT TGT Enterococcus gilvus ATCC BAA-350 144 61°C 40 Clostridium leptum GCA CAA GCA GTG GAG T CTT CCT CCG TTT TGT CAA Clostridium leptum ATCC 29065 239 60°C 41 Bacteroides ATA GCC TTT CGA AAG RAA GAT CCA GTA TCA ACT GCA ATT TTA Bacteroides vulgatus ATCC 8482 495 60°C 42 Prevotella CAC RGT AAA CGA TGG ATG CC GGT CGG GTT GCA GAC C Prevotella nigrescens ATCC 33563 513 55°C 42 Bifidobacterium CGC GTC YGG TGT GAA AG CCC CAC ATC CAG CAT CCA Bifidobacterium longum subsp. Infantis ATCC 15697 244 60°C 39 Open in a separate window AT, annealing temperature; bp, base pairs.

Techniques: Real-time Polymerase Chain Reaction, Sequencing